Effect of the bioproduct Lebame® on the creaminess of *Oryza sativa* L. (cultivar IACuba 41) grains.

Authors

DOI:

https://doi.org/10.62452/pdvm2t78

Keywords:

Biostimulants, endosperm, efficient microorganisms, industrial quality, milling yield

Abstract

The commercial quality of rice (Oryza sativa L.) is compromised by appearance defects such as endosperm chalkiness, which reduces milling yield. This study evaluated the effect of the Lebame® bioproduct on the percentage of chalky grains in the IACuba 41 cultivar. A field experiment was established on a Vertisol, analyzing seed imbibition with the bioproduct at a dose of 10 mL L⁻¹ for 24 hours, followed by 48 hours of shaded aeration prior to sowing, plus two field applications at 25 and 50 days after sowing. An independent-samples Student's t-test demonstrated that 24-hour imbibition at 10 mL L⁻¹ significantly reduced the incidence of this defect relative to the treatment without the bioproduct. The Bayes factor and bootstrap analysis confirmed the robustness of the difference (p < 0.001). The control group recorded a mean of 26.30 % with greater dispersion, whereas the experimental treatment reached a mean of 15.10 %, representing an absolute decrease of 11.20 percentage points. The findings indicated that the synergistic action of the microorganisms favored endosperm filling. The study concluded that this biostimulant represents a viable alternative for optimizing the apparent quality and industrial value of the grain.

Downloads

Download data is not yet available.

References

Alfonso-Carballo, R., Rivero-Landeiro, L. E., Suárez-Crestelo, E., Martínez-Grillo, J., Riverón-Valdés, A., Cabañas-Hecgavarría, M., Gonzélez-Morera, T., Alemán-Manfarrol, L., Adelfa-Hernández, A., Contreas-Pérez, Y., Hong-Son, N., Truong-Son, Y., Xuan-Dung, N., & Thi-Van, T. (2020). Instructivo técnico del cultivo del arroz. Instituto de Investigaciones de Granos, Ministerio de la Agricultura.

Carrillo-Sosa, Y., Terry-Alfonso, E., Ruiz-Padrón, J., Díaz-De Villegas, M. E., & Delgado, G. (2017). Efecto del LEBAME en la germinación de semillas de tomate (Solanum lycopersicum L.). Cultivos Tropicales, 38(3), 30–35. https://ediciones.inca.edu.cu/index.php/ediciones/article/view/4/2065

Casanovas, E., Suárez del Villar, A., Álvarez, A., & Avilleira, I. (2022). Valoración de la seguridad alimentaria cubana a partir de la superficie agrícola explotada y los rendimientos agrícolas. Revista Universidad y Sociedad, 14(5), 304–314. https://rus.ucf.edu.cu/index.php/rus/article/view/3226

Chowdhury, N. B., Chandran, A. K. N., Walia, H., Shahreen, N., Stone, E., Knobbe, E., & Saha, R. (2025). Transcriptome enhanced rice grain metabolic model identifies histidine level as a marker for grain chalkiness. Scientific Reports, 15, Article 16432. https://doi.org/10.1038/s41598-025-00504-6

Comisión Económica para América Latina y el Caribe. (2024). Perspectivas de la agricultura y el desarrollo rural en América Latina y el Caribe 2023–2024: Una mirada hacia América Latina y el Caribe. IICA. https://repositorio.cepal.org/server/api/core/bitstreams/6eed817e-f9c7-449a-913f-76bb41c13375/content

Doni, F., Suhaimi, N. S. M., Mispan, M. S., Fathurrahman, F., Marzuki, B. M., Kusmoro, J., Isahak, A., & Uphoff, N. (2022). Microbial contributions for rice production: From conventional crop management to the use of 'omics' technologies. International Journal of Molecular Sciences, 23(2), 737. https://doi.org/10.3390/ijms23020737

Gómez-Kosky, R., Bernal-Villegas, A., Reyes-Esquirol, C. F., Núñez-Jaramillo, D., Bermúdez-Calimano, M., Cárdenas-Cuellar, Y., Machado-Armas, P., & Castillo-León, R. (2019). Efecto de los microorganismos eficientes Lebame, en la aclimatización ex vitro de caña de azúcar (Saccharum spp.) cultivar C87-51. Revista ICIDCA sobre los Derivados de la Caña de Azúcar, 53(3), 3–12. https://www.revista.icidca.azcuba.cu/wp-content/uploads/2020/05/art%C3%ADculo-2-2.pdf

He, C., Deng, F., Yuan, Y., Huang, X., He, Y., Li, Q., Li, B., Wang, L., Cheng, H., Wang, T., Tao, Y., & Wang, L. (2024). Appearance, components, pasting, and thermal characteristics of chalky grains of rice varieties with varying protein content. Food Chemistry, 440, 138256. https://doi.org/10.1016/j.foodchem.2023.138256

International Rice Research Institute, & International Network for Genetic Evaluation of Rice. (2014). Standard evaluation system for rice (SES) (5th ed.). International Rice Research Institute.

Jeffreys, H. (1961). Theory of probability (3rd ed.). Oxford University Press.

Kumawat, A., Kumar, D., Shivay, Y. S., Bhatia, A., Rashmi, I., Yadav, D., & Kumar, A. (2023). Long-term impact of biofertilization on soil health and nutritional quality of organic basmati rice in a Typic Ustchrept soil of India. Frontiers in Environmental Science, 11, 1031844. https://doi.org/10.3389/fenvs.2023.1031844

Nakamura, S., Satoh, A., Aizawa, M., & Ohtsubo, K. (2022). Characteristics of physicochemical properties of chalky grains of Japonica rice generated by high temperature during ripening. Foods, 11(1), 97. https://doi.org/10.3390/foods11010097

Ortega-Arias-Carbajal, G. M., Díaz de Villegas, M. E., Delgado-Arrieta, G., & Martínez-Sánchez, A. (2015). Estudio de estabilidad del bioproducto Lebame. Revista ICIDCA sobre los Derivados de la Caña de Azúcar, 49(3), 3–8. https://www.redalyc.org/pdf/2231/223144218001.pdf

Pacheco, I., Ferreira, R., Correia, P., Carvalho, L., Dias, T., & Cruz, C. (2021). Microbial consortium increases maize productivity and reduces grain phosphorus concentration under field conditions. Saudi Journal of Biological Sciences, 28(1), 232–237. https://doi.org/10.1016/j.sjbs.2020.09.053

Park, J.-R., Ha, S.-K., Lee, H.-S., Lee, G., Lee, S. Y., Kang, K. M., Suh, J.-P., Jin, M., Park, H.-S., Lee, C.-M., Seo, J., Park, S., Lee, K.-M., & Jeong, O.-Y. (2025). Climate-driven trends in rice grain appearance: A 2023–2024 comparative study using Korea field data. Journal of Crop Science and Biotechnology, 28(5), 657–668. https://doi.org/10.1007/s12892-025-00308-0

R Core Team. (2026). R: A language and environment for statistical computing. R Foundation for Statistical Computing. https://www.r-project.org/

Ríos-Ruiz, W. F., Torres-Chavez, E. E., Torres-Delgado, J., Rojas-Garcia, J. C., Bedmar, E. J., & Valdez-Nunez, R. A. (2020). Inoculation of bacterial consortium increases rice yield (Oryza sativa L.) reducing applications of nitrogen fertilizer in San Martin region, Peru. Rhizosphere, 14, 100200. https://doi.org/10.1016/j.rhisph.2020.100200

Thongnok, S., Siripornadulsil, W., Thanwisai, L., & Siripornadulsil, S. (2024). As(III)-oxidizing and plant growth-promoting bacteria increase the starch biosynthesis-related enzyme activity, 2-AP levels, and grain quality of arsenic-stressed rice plants. BMC Plant Biology, 24, Article 672. https://doi.org/10.1186/s12870-024-05352-6

Tokatlidis, I. S., Vrochidis, I., Sistanis, I., Pankou, C. I., Sinapidou, E., Papathanasiou, F., & Vlachostergios, D. N. (2023). Testing the validity of CV for single-plant yield in the absence of competition as a homeostasis index. Agronomy, 13(1), 176. https://doi.org/10.3390/agronomy13010176

Wang, H., Xiong, R., Zhou, Y., Tan, X., Pan, X., Zeng, Y., Huang, S., Shang, Q., Xie, X., Zhang, J., & Zeng, Y. (2022). Grain yield improvement in high-quality rice varieties released in southern China from 2007 to 2017. Frontiers in Sustainable Food Systems, 6, 986655. https://doi.org/10.3389/fsufs.2022.986655

Zambrana, J. M. F., Maqueira López, L. A., Pérez León, N., & Páez López, A. (2025). Productividad del cultivar de arroz IACuba 41, en área de producción de semilla en Los Palacios. Avances, 27(2), 166–178. https://dialnet.unirioja.es/servlet/articulo?codigo=10117642

Zhao, D., Sreenivasulu, N., & Liu, Q. (2026). Mitigating rice chalkiness while ensuring grain quality and seed vigor. Plant Communications. https://doi.org/10.1016/j.xplc.2026.101816

Downloads

Published

2026-10-30

How to Cite

Cano-Llorente, S., Rodríguez-Rodríguez, S. F., Chávez-Cárdenas, M. del C., Tamayo-Saborit, M., Linares-Álvaro, M. J., & Cueto-Aldana, G. (2026). Effect of the bioproduct Lebame® on the creaminess of *Oryza sativa* L. (cultivar IACuba 41) grains. Revista Metropolitana De Ciencias Aplicadas, 9(6), 5-14. https://doi.org/10.62452/pdvm2t78