Evaluación de la sensibilidad de Phytophthora infestans (Mont) Bary al fungicida Oxatiapiprolin
Resumen
El manejo de cepas más agresivas de Phytophthora infestans es un desafío para México, por lo que se propuso como objetivo validar y monitorear la eficacia de (Oxatiapiprolin), frente a la sensibilidad de micropoblaciones de aislados de campo de P. infestans, aplicando un protocolo con discos de hojas ajustado previamente con cinco concentraciones del fungicida a presión de 1,2 bar en un aislado severo P. infestans del Valle de Toluca, Estado de México. El fungicida mostró un importante efecto inhibitorio sobre un aislado mixto de P. infestans en condiciones in vitro, con las cinco primeras concentraciones usadas en el test (1-0,0016 ppm). A una mayor concentración del fungicida, se produjo un aumento de la sensibilidad del patógeno P. infestans y por tanto una menor afectación del tejido, que hasta la 5a concentración se produce una afectación menor al 25% del tejido (0–2,6 mm), comparado con el daño del testigo (13 mm) (>95 %) cuando no se aplica el fungicida. La inhibición media (EC50) es alta en la concentración aproximada de 0,00034 mgL-1 del fungicida, que inhibe el 50% del crecimiento poblacional del patógeno.
Palabras clave:
Oomicetes, tizón tardío, resistencia, fitopatógeno, inhibición.
ABSTRACT
The management of aggressive strains of Phytophthora infestans is a challenge for Mexico, so the objective of study proposed to validate and monitor the efficacy of (Oxatiapiprolin), against the sensitivity of P. infestans micropopulations, isolated from the field. A modified protocol with leaf discs and five concentrations of the fungicide at a pressure of 1.2 bar was tested against a severe late blight isolate from the Toluca Valley, State of Mexico. The fungicide showed an inhibitory effect on a mixed isolate of P. infestans under in vitro conditions, compared to the first five concentrations used in the test (1-0.0016 ppm). It was observed that at a higher concentration, there was an increase in the sensitivity of the pathogen and less damage to the tissue. After 5th concentration, less than 25% of tissue was affected (0–2.6 mm) compared with the damage in control (13 mm) (>95%), when the fungicide was not applied. The mean inhibition (EC50) is high calculated at approximately 0.00034 mgL-1 of the fungicide, which inhibits 50% of the growth of the pathogen.
Keywords:
Oomycetes, late blight, resistance, phytopathogen, inhibition.
Texto completo:
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ISSN on line: 2631-2662
ISSN impreso: 2661-6521